Article Abstract:
An analysis the cAMP-mediated ATP efflux from Saccharomyces cerevisiae was conducted to determine ATP's important role in the physiology of multicellular organisms. Results show that ATP efflux require glucose. Furthermore, the rate of efflux was increased by proton ionophores such as nigericin and monensin as well as by the plasma membrane proton pump inhibitor diethylstilbestrol. Results also indicat that the cAMP pathway triggered ATP efflux and that yeast cells release ATP in a regulated manner.
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Article Abstract:
A study was conducted to analyze carbon distribution from glucose and glutamate using anaerobically grown Saccharomyces cerevisiae. A Chemap CMF200 bioreactor equipment was utilized to prepare cultures while the biomass concentration was determined by OD(sub 610) measurements. Results showed that the carbon flow was characterized by two separate paths. Findings also indicated that only parts of the tricarboxylic acid cycle were utilized when glutamate was in the nitrogen source.
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Article Abstract:
Deletion of the NHA1 gene of Saccharomyces cerevisiae wild type strains, lead to higher sensitivity to both K+ and Na+ at acidic pH. High K+ and Na+ efflux seen on alkalinization of the cytoplasm suggests a role for Nha1p in the regulation of intracellular pH. The Nha1 alkalication antiporter is found to be responsible for growth on high concentrations of KC1 and NaC1.
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