Genotypic and phenotypic analysis of zwittermicin A-producing strains of Bacillus cereus

Article Abstract:

A zwittermicin A-resistance gene was investigated using fatty acid analysis, RAPD analysis and PCR amplification to identify strains that produce zwittermicin A from other Bacillus cereus strains. According to results of a cluster analysis of the fatty acid methyl ester profiles, zwittermicin A producers grouped together in two clusters, apart from most non-producers. Like FAME, PCR analyses also distinguished B. cereus strains that produce zwittermicin A from other strains. It was also found that PCR with the primers designed to amplify zmaR is the most reliable method among those tested for the identification of zwittermicin A producers.

author: Handelsman, Jo, Stabb, Eric V., Raffel, Sandra J., Milner, Jocelyn L.
Analysis, Antibiotics, Bacillus cereus

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Genotypic analysis of Mycobacterium tuberculosis from medieval human remains

Article Abstract:

The DNA sequences of three medieval bone samples with osteological evidence of tuberculosis infection were found to be similar to those found in present-day Mycobacterium tuberculosis. This was gleaned from a series of polymerase chain reaction analyses of the medieval bone samples in which infection with a bacterium belonging to the Mycobacterium tuberculosis complex was identified. Spoligotyping provided further evidence of similarity between Mycobacterium tuberculosis isolates and the infective strain from the bone samples.

author: Young, D., Taylor, G.M., Goyal, M., Legge, A.J., Shaw, R.J.
Bones, Tuberculosis, Mycobacterium tuberculosis, Polymerase chain reaction, Bacterial genetics, Anthropometry, Amino acid sequence, Amino acid sequencing

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Carbapenems as inhibitors of OXA-13, a novel, integron-encoded beta-lactamase in Pseudomonas aeruginosa

Article Abstract:

The induction of beta-lactamases confers resistance to beta lactam antibiotics on pathogenic bacteria. Pseudomonas aeruginosa is notorious for its antibiotic resistance because of the presence of a cephalosporinase gene in its genome making it naturally resistant to cephalotin and ampicillin. A clinical strain of P. aeruginosa has been found to contain an oxacillinase inhibited by imipenem.

author: Mugnier, P., Podglajen, I., Goldstein, F.W., Collatz, E.
Usage, Physiological aspects, Pseudomonas aeruginosa, Beta lactamases, Imipenem

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subjects list: Research, Genetic aspects, Drug resistance in microorganisms, Microbial drug resistance
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