Article Abstract:
Restriction fragment length polymorphism (RFLP) analysis was conducted on 10 stream isolates of Janthinobacterium lividum, an ubiquitous purple bacterium, using a rRNA gene as a probe. The results showed that J. lividum exhibits a high degree of genetic variation, as seen in terms of nucleotide diversity, with a value of 0.0781. This high degree of variation could be reflective of a high degree of genetic recombination, which could occur along the longitudinal gradient of the stream. The RFLP analysis also yielded possible molecular markers for J. lividum.
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Article Abstract:
PCR was employed to develop a library of 16S rRNA genes cloned from DNA extracted from the sediments of Rainbow Bay, situated on the Savannah Riber Site, near Aiken, SC. Of the 35 clones sequenced, 32 were related to five bacterial groups: 11 clustered with the Proteobacteria division, eight clustered with the Acidobacterium subdivision of the Fibrobacter division, seven clustered with the Verrucomicrobium subdivision of the Planctomyces division, three clustered with the gram-positive bacteria, and 3 clustered with the green nonsulfur bacteria.
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Article Abstract:
The allelic variation in a population of Burkholderia cepacia isolated from a blackwater creek was studied by using the multilocus enzyme electrophoresis technique. In Burkholderia both clonality and sexuality are seen. The mean genetic variation in the population studied, was found to be 0.574. The bacteria found in the soil at the same spot did not show much variation. Those found from different places differed genetically. This shows that the bacteria adapt themselves to changes in their micro environment.
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