Article Abstract:
The transfer of the plant pathogenic bacteria Erwinia amylovora ams genes and E. stewartii cps genes into the mutants of other species that are unable to produce exopolysaccharides (EPS) stimulates the production of stewartan and amylovoran, respectively. However, the E. stewartii cpsK mutant complemented with ams genes produces stewartan. Both the EPSs are acylated in E. amylovora but not in E. stewartii. The synthesis of amylovoran in E. stewartii restores its virulence, but stewartan is unable to do so in E. amylovora.
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Article Abstract:
A 0.9-kb fragment of plasmid pEA29, which is common to all strains of Erwiniaamylovora, was selectively amplified using the polymerase chain reaction, resulting in a sensitive method for detecting E. amylovora, the fire blight pathogen. The plasmid and its marker are highly specific to E. amylovora, whichwas confirmed by hybridization test on other bacterial species. The procedure also eliminated the DNA hybridization step by using the specific signal recognized in electrophoresis of the amplified sample on a gel.
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Article Abstract:
The fire blight pathogen, Erwinia amylovora is individually identified from the common plasmid pEA29 using three discrete polymerase chain reaction (PCR) assays by chromosomal DNA. Two primer PCR assays having information from the ams genes confirm the sequence which are same for two E. amylovora strains. The sequence of the 16S rRNA resembles the sequences of E. amylovora and other Erwinia species.
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