Article Abstract:
The pcbC and pcbD genes of Pseudomonas sp. strain DJ-12 encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase and 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid hydrolase, respectively, are present as an operon. The pCU103 plasmid contains an open reading frame (ORF) at position 82 which is the pcbD gene with 849 bp and another ORF of 960 bp from position 1022 is the pcbC gene. A promoter-like sequence is located upstream of the pcbD gene and ribosome-binding sites are present upstream of the initiation codons of pcbC and pcbD genes.
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Article Abstract:
A study was conducted to investigate the mechanism involved in Mn2+ oxidation of Pseudomonas putida strain GB-1. Nucleotide sequence analysis of the transposon insertion site showed that a gene encoding a protein similar to multicopper oxidases is involved in Mn2+ oxidation. This gene was labeled a cumA. It was also found that GB-1 strain and other Mn2+-oxidizing speodomonads need Cu2+-binding proteins to facilitate Mn2+ oxidation in Pseudomonas putida.
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Article Abstract:
A self-formed adaptor PCR (SEFA PCR) that can be used for chromosome walking was developed. Most of the amplified flanking sequences were found to be longer than 2.0kb and some were 6.0 kb which suggests that SEFA PCR is a simple and efficient method for the isolation of unknown sequences in complex genomes.
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