Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes

Article Abstract:

A nicaraguan field isolate (SfNIC) of spodoptera frugiperda nucleopolyhedrovirus was purified by plague assay on Sf9 cells and nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. It is concluded that the SfNIC population presents high levels of genetic diversity, localized to a 17-kb, region containing pif and pif-2.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Optical brighteners do not influence covert baculovirus infection of Spodoptera frugiperda

Article Abstract:

The influence of the presence of an optical brightener (Tinopal UNPA-GX) on covert infection with Spodoptera frugiperda multiple nucleopolyhedrovirus is examined by reverse transcription PCR of virus gene transcripts. The analysis shows that the detection of the covert infection is dependent on insect life stage and the viral mRNA used for diagnosis.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Functional importance of deletion mutant genotypes in an insect nucleopolyhedrovirus population

Article Abstract:

A Nicaraguan isolate of a nucleopolyhedrovirus (SfNIC) attacks the fall armyworm Spodoptera frugiperda, survives as a mixture of nine genotypes (SfNIC A to I) that all present genomic deletions, except variant B (complete genotype). The result demonstrates the value of retaining genotypic diversity in virus-based bioinsecticides.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: