Genetic localization and regulation of the maltose phosphorylase gene, MaIP, in Lactococcus lactis

Article Abstract:

The maltose phosphorylase (MP) gene, MaIPGenetic, and its localization and regulation in Lactococcus lactis have been studied. An effect of glucose on MP expression was shown by carrying out fermentations in the presence of maltose and glucose. The genetic locus of the MP gene was in the area of the region that encodes a possible regulator in the Lacl-GalR family of transcriptional regulators. Different genetic loci for the genes that encode MP and beta-phosphoglucomutase account for the different gene regulation behavior.

author: Nilsson, Ulrika, Radstrom, Peter
Sweden, Statistical Data Included, Cytochemistry, Molecular biology, Glucose, Lactococcus, Phosphorylase

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Determination of the pathway for rhamnose biosynthesis in mycobacteria: cloning, sequencing and expression of the Mycobacterium tuberculosis gene encoding alpha-D-glucose-1-phosphate thymidylyltransferase

Article Abstract:

The gene rfbA gene that encodes the alpha-D-glucose-1-phosphate thymidylyltransferase (RfbA) of Mycobacterium tuberculosis was analyzed to determine the genetic mechanisms that mediate the biosynthesis of rhamnose. Analysis of rfbA gene sequence indicated the presence of an ATG start codon with a 31362 Da putative protein product that was similar to other microbial RfbA proteins. Furthermore, other rfbA genes required for rhamnose biosynthesis was not detected during the sequencing of DNA surrounding the rfbA gene.

author: Ma, Yufang, Mills, Jonathan A., Belisle, John T., Vissa, Vara, Howell, Mark, Bowlin, Kelly, Scherman, Michael S., McNeil, Michael
Mycobacterium tuberculosis

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Product formation and phosphoglucomutase activities in Lactococcus lactis: cloning and characterization of a novel phosphoglucomutase gene

Article Abstract:

The biochemical characteristics and molecular structure of the gene encoding beta-phoshoglucomusate (beta-PGM) which mediates maltose metabolism was analyzed in Lactococcus lactis. Analysis of pgmB gene from the Lactococcus lactis genomic library indicated the presence of a 24210 Da polypeptide that was expressed by the beta-PGM gene. Furthermore, the expression of pgmB gene in Lactococcus lactis was inhibited by the presence of lactose.

author: Stanley, Grant A., Radstrom, Peter, Qian, Ny, Bunte, Annicka
Bacteria, Lactose

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subjects list: Genetic aspects, Microbiological synthesis, Microbial enzymes
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