Article Abstract:
Resistance to anti-microtubule herbicides of Chlamydomonas reinhardtii mutations arising from genetic interactions in the aminoprophos-methyl (APM) 1 and APM2 loci were further studied. Additional allele-specific genetic interactions were discovered in both loci aside from the allele-specific lethality and unlinked noncomplementation interactions. Flagellar regeneration was found to be inhibited by these mutations. The genetic interactions among the gene mutations indicated modified stability of the complex wherein mutant products were found active.
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Article Abstract:
The unicellular eukaryote Chlamydomonas reinhardtii features flagella and a chloroplast. Studies on Chlamydomonas are important in determining the protein components of the basal body and determining their functions in organelles. Several methods have been developed to duplicate Chlamydomonas genes wherein mutations generate novel and interesting phenotypes. Three of the most popular methods are insertional mutagenesis using plasmids, cloning by complementation and positional cloning.
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Article Abstract:
Researchers isolated the NIT2 gene of Chlamydomonas reinhardtii, employing a transposon tagging plan, to understand more about the role of the NIT2 gene product. The NIT2 gene encodes a 6-kb transcript that was not found in cells developed in the presence of ammonium. The repression of the NIT2 gene may mediate metabolite repression of the nitrate assimilation passage in Chlamydomonas.
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