Article Abstract:
Streptomyces rimosus R6 exhibits a high level of morphological instability, as reflected in sporulation, pigmentation, colony morphology, oxytetracycline production and oxytetracycline resistance phenotypes. Based on oxytetracycline resistance, three classes of variants were observed. Pulsed-field gel electrophoresis showed that most of these variants exhibit a reproducible large-scale DNA rearrangement, which probably included a deletion and a low-level repetition of a DNA fragment. The implications of these results in strain degeneration and antibiotic overproduction are discussed.
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Article Abstract:
A physical map of the Streptomyces rimosus R6-501 8 Mb linear chromosome and its oxytetracycline (OTC) gene cluster was analyzed based on standard molecular genetic techniques. Analysis of the Streptomyces rimosus R6-501 8 Mb linear chromosome indicated the presence of 550 kb inverted repeats and a possible transposable element that mediated the formation of a long inverted repeat structure. Furthermore, the genetic organization of Streptomyces rimosus was similar to the genome map of Streptomyces coelicolor A3 strains.
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Article Abstract:
Genetics of Streptomyces rimosus, the producer of oxytetracyclin killer, is investigated. The integrated genomic data provided by the study presents a platform to undertake their manipulation of the S. rimosus chromosome, informed by observations from other species, which increase antibiotic productivity, in a well-defined way.
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