Genetic characterization and transmission cycles of Cryptosporidium species isolated from humans in New Zealand

Article Abstract:

Genetic difference was identified among the Cryptosporidium species from rural and urban isolates in New Zealand, the Cryptosporidium hominis was more common in the urban area and the Cryptosporidium parvum bovine genes were more common among the rural areas. It was further noticed that the transmission cycles also changed like the anthroponotic cycle in autumn and the zoonotic cycle in spring.

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Validation of a PCR-based method for detection of food-borne thermotolerant campylobacters in a multicenter collaborative trial

Article Abstract:

A technique dependent on the PCR for speedily identifying the food-borne thermotolerant campylobacters was analyzed thoroughly in a collaborative trial at 12 laboratories, which tested the spiked carcass rinse models. A complete standard operating procedure (SOP) that specified all the details about how to conduct the pre-PCR treatment and the PCR assay were supplied to all the 12 laboratories.

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Intraspecies polymorphism of Cryptosporidium parvum revealed by PCR-restriction fragment length polymorphism (RFLP) and RFLP-single-strand conformational polymorphism analyses

Article Abstract:

The authors have investigated Cryptosporidium parvum glycoprotein-encoding gene. The use of this gene for identification and gene fingerprinting of C. parvum isolates via PCR-restriction fragment length polymorphism-single-strand conformational polymorphism and PCR-restriction fragment length polymorphism methods is described.

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