Genetic analysis of developmental mechanisms in hydra: XX. Cloning of interstitial stem cells restricted to the sperm differentiation pathway in Hydra magnipapillata

Article Abstract:

A study was conducted on Hydra magnipapillata polyps whose interstitial stem cells were capable of differentiating only into sperm and not into somatic cells. Clones of these pseudo-epithelial hydra were obtained and maintained through force-feeding. All specimens developed interstitial cells during the study period but produced neither nematocytes or nerve cells. Instead, all interstitial cells developed into gametic cells. These results suggest that Hydra magnipapillata's interstitial stem cell population has a subpopulation whose cells are limited to the germ line differentiation pathway.

author: Sugiyama, Tsutomu, Nishimiya-Fujisawa, Chiemi
Gametogenesis, Leydig cells

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Two types of female germ stem cells are present in a male strain of Hydra magnipapillata

Article Abstract:

The interstitial stem cell subpopulations in the male strain of hydra, Hydra magnipapillata, are described according to their roles in sex determination. The nem-1 strain contains egg-restricted and multipotent stem cells, which are both capable of differentiating into eggs but not into sperms. As a result, although male in phenotype, the strain differentiates into eggs. However, differentiation is suppressed during sex induction. Hydra that contain these kinds of stem cells are called phenotypic males and are believed to be similar to hermaphroditic hydra.

author: Sugiyama, Tsutomu, Nishimiya-Fujisawa, Chiemi
Genetic aspects, Stem cells, Hermaphroditism

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Patterning of heads and feet during regeneration of Hydra oligactis aggregates

Article Abstract:

A study was conducted on the de novo appearance and spacing of head organizing centers during aggregate regeneration in specimens made from dissociated Hydra oligactis body column tissue using CP8. Within 24 hours of regeneration, newly differentiated CP8+ head organizing centers appeared. Some subsequently developed into heads or tentacles, while others remained as patches. The number of original CP8+ patches and final CP8+ structures was found to be constant, indicating that during regeneration, a limited amount of head organizing centers are formed.

author: Pei-Chieh Lee, Javois, Lorette C.
Regeneration (Biology)

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subjects list: Research, Cell differentiation, Hydra, Hydras
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