Article Abstract:
Cells of Saccharomyces cerevisiae were chemically mutagenized and screened for mutants lacking acidity in the vacuole. Of the resulting 39 mutants, eight corresponded to new loci affecting the vacuolar pH (vph). These loci were named VPH1, VPH2 and VPH4-VPH9. In addition, four mutant alleles corresponded to the previously described PEP3, PEP5, PEP6 and PEP7 genes, which affect carboxypeptidase Y activity. These results suggest a certain degree of overlap between the VPH and PEP functions.
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Article Abstract:
A study was conducted to analyze mutS/hexA homologs from Saccharomyces cerevisiae as mismatch repair proteins. Amplification and cloning of these homologs was done through degenerate oligonucleotide primers based on E coli MutS protein regions and its homologs in polymerase chain reaction. A pair of DNA sequences were then amplified and used to clone genes from a yeast genomic library. Results show that the MutS homolog genes functioned in mitochondrial and nuclear DNA repair.
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Article Abstract:
A study was conducted to evaluate MSH1 and MSH2 genes of Saccharomyces cerevisiae. These genes are assumed to encode proteins homologous to Escherichia coli MutS and Streptoccocus pneumoniae proteins and relative homologs. Disrupted MSH1 genes generate a rapidly developing phenotype. Results show that MSH1 functions in mitochondrial DNA repair or stability while MSH2 functions in 4-bp insertion/deletion mismatched repairs.
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