Article Abstract:
Three yeast genes, CDC16, CDC23 and CSE1, have been found to be involved in the proteolysis of cyclin CLB2, a B-type cyclin, during the G1 phase and anaphase of cell division. Proteolysis of other unstable proteins such as SW15 and CLB5 is not dependent on these genes. CLB2 proteolysis starts at the initiation of anaphase, but a fraction of CLB2 does not undergo proteolysis and remains stable till the end of anaphase. The function of CSE1 is not known, while CDC23 and CDC16 appear to be involved in sister chromatid separation during the transition from metaphase to anaphase, which may involve proteolysis, and in cyclin destruction during anaphase and G1.
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Article Abstract:
Chromatid cohesion among sister chromatids as studied to identify the proteins involved in the process using fluorescent in situ hybridization. Mutants that lose chromosome which are capable of separating sister chromatids without an anaphase promoting complex were isolated. Results show that proteins such as Smc1p, Smc3p, Scc1p and Scc2p are involved in sister chromatid cohesion. Separation of sister chromatids in the anaphase stage of mitotic division is believed to be the cause of loss of cohesion where Scc1p dissociates from chromatin and failure of chromatids to align in metaphase results to tumorigenesis.
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Article Abstract:
The separation of sister chromatids between metaphase and anaphase is mediated by the dissociation of the cohesin protein Scc1p. The dissociation of Scc1p from the chromatids is made possible by the liberation of the ESP1 protein from the ESP1/PDS1 complex mediated by the anaphase promoting complex (APC). APCs promote the dissociation of sister chromatids by liberating ESP1 and not by degrading Scc1p.
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