Genes and enzymes of the acetyl cycle of arginine biosynthesis in the extreme thermophilic bacterium Thermus thermophilus HB27

Article Abstract:

An arginine biosynthetic gene cluster, argC-argJ, of the extreme thermophilic bacterium Thermus thermophilus HB27 was isolated. The recombinant plasmid (pTHM1) provided ornithine acetyltransferase activity to the Escherichia coli host, suggesting that T. thermophilus employs the pathway which requires less energy for the deacetylation of acetylornithine. However, pTHM1 could not complement an E. coli argA mutant and no acetylglutamate synthase activity was observed in E. coli argA cells with pTHM1.

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Two amino acid amidohydrolase gene encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus

Article Abstract:

The cotranscription of the Bacillus stearothermophilus L-carbamoylase gene and L-aminoacylase gene as a single mRNA occurs from the same transcriptional start. The Bacillus stearothermophilus strains conserve this two-ama-gene operon. The cross-activity of L-carbamoylase towards the substrates for L-aminoacylase suggests a common ancestor and a genetic and functional relationship between the two amino acid amidohydrolase genes.

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Genes and enzymes of the acetyl cycle of arginine biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early steps of the arginine pathway

Article Abstract:

Previous research has failed to clarify the organization and regulation of the acetylation pathway in the arginine acetyl cycle. To remedy this problem, a genetic and enzymic examination of the pathway in the Gram-positive mesophilic bacterium, Corynebacterium glutamicum, genetically improved strains of which have long been used to produce arginine and proline, is undertaken.

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