Article Abstract:
The effect of electrophiles on the metabolic activity of Pseudomonas putida was investigated using N-ethylmaleimide (NEM) as a model synthetic electrophile. Cells exposed to NEM exhibited reduced growth followed by recovery from exposure. Subsequent exposures to NEM caused cells to develop resistance to NEM, suggesting the accelerated detoxification of NEM. Cultures grown on minimal agar showed a transient loss of ability to form colonies. In contrast, cells grown on MacConkey agar regained full viability after NEM exposure. Since peptones are the active component in MacConkey agar, it was suggested that peptones accelerate the detoxification of NEM.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
A study was conducted to investigate the influence of the biodegradation kinetics of toluene, m-xylene, p-xylene and their intermediates through the Pseudomonas putida pathway. Cells were determined from chemostat cultures prepared under either m-xylene or toluene limitation. Results indicated that the first monooxygenation step is the slowest step of the upper pathway in vivo. Findings also showed that that pathway can be utilized in characterizing the disappearance of various compounds in terms of the degrading population.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
Strain AN103 of Pseudomonas fluorescens is a soil bacterium that was isolated because of its ability to grow with only ferulic acid as an energy and carbon source. The catabolism of ferulic acid in this strain involves stepwise degradation yielding three intermediates, vanillin, vanillic acid and protocatechuic acid. The catabolic pathway was shown to require the enzyme ferulate-CoA ligase and CoASH, ATP and magnesium chloride as cofactors.
User Contributions:
Comment about this article or add new information about this topic: