Gene silencing: repeats that count

Article Abstract:

Repeat-induced-point mutation (RIP) is a gene silencing process that arises from direct DNA-DNA interactions in the fungus Neurospora crassa. Methylation-induced premeiotically (MIP) is a similar process that occurs in the fungus Ascobolus immersus, the difference between the two being that gene silencing by MIP does not involve mutations. MIP and RIP are unique since they are the only known silencing mechanisms that involve the counting of repeats. However, there is some uncertainty about the role played by RNA-RNA interactions and RNA-DNA interactions in these silencing mechanisms.

author: Selker, Eric U.
Genetic aspects, Eukaryotic cells, Cells (Biology), Eukaryotes, Fungi, RNA

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Neurospora and the molecular revolution

Article Abstract:

Modern biochemical genetics started from George Beadle and Edward Tatum's experiments on biochemical mutants of the bread mold Neurospora. The study showed that a single gene correlates with one specific enzyme which later became known as the one-gene-one-enzyme principle. The discipline, which was based on conventional genetics, was further advanced by the identification of DNA genes and the introduction of microorganisms into genetical research.

author: Horowitz, Norman H.
Molecular genetics

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Ectopic integration of transforming DNA is rare among neurospora transformants selected for gene replacement

Article Abstract:

Transformants with multiple copies of the transforming DNA are produced in several organisms by the DNA mediated transformation. The transformants include unselected and selected molecules. The formation of these transformants reveals that the subpopulations of nuclei or cells are competant for transformation. The transformation occur by homologous recombination of the DNA molecules.

author: Selker, Eric U., Miao, Vivian P. W., Rountree, Michael R.

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subjects list: Research, DNA, Neurospora
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