Gene cluster responsible for validamycin biosynthesis in Streptomyces hygroscopicus subsp. jinggangensis 5008

Article Abstract:

A gene cluster responsible for the biosynthesis of validamycin (Val), an aminocyclitol antibiotic widely used as a control agent for sheath blight disease of rice plants, was identified from Streptomyces hygroscopicus subsp. jinggangensis 5008. The direct involvement of valA, in validamycin biosynthesis was confirmed in vivo by gene inactivation as well as in vitro by biochemical characterization of the reaction catalyzed by the ValA protein heterologously overexpressed in Escherichia coli.

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Inhibition of epsilon-poly-L-lysine biosynthesis in Streptomycetaceae bacteria by short-chain polyols

Article Abstract:

A study was conducted to identify the unknown derivative as the ester formed between the hydroxyl group of a glycerol molecule and the terminal carboxyl group of an epsilon-poly-L-lysine (ePL) molecule, secreted by Streptomycetaceae bacteria, using matrix-assisted laser desorption ionization time of flight mass spectroscopy and nuclear magnetic resonance. The results revealed that the number of lysine residues of ePL-polyol esters decreased with the increasing polyol concentration.

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Purification, characterization and sequencing of an extracellular cold-active aminopeptidase produced by marine psychrophile Colwellia psychrerythraea strain 34H

Article Abstract:

A description is given of the purification and initial biochemical and structural characterization of a cold-active, extracellular, family M1 aminopeptidase produced by Colwellia psychrerythraea strain 34H. The potentiality of the stabilizing effect on Co1AP of extracellular polymeric substances is also examined.

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