Article Abstract:
A new strategy that merges the different community analysis approaches by combining GC fractionation and denaturing gradient gel electrophoresis (DGGE) methods is used to enhance the assessment of the microbial community diversity and detect the minority populations of the microbes. The combination of GE fractionation and DGGE method overcomes the individual limitations of each method and permits visualization and recovery of sequences from the minority populations of bacteria.
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Article Abstract:
A study was conducted to analyze a DNA-based, direct technique for the initial preparation of the total bacterial community in ileum and cecum of the gastrointestinal tract of chickens. Total bacterial DNA samples were determined based on the percent G+C content. The digesta samples were kep on ice and processed within two hours. Experimental results indicated the effectiveness of the technique in examining the culturability of the bacteria.
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Article Abstract:
The composition of gastrointestinal microflora in chickens can be controlled by diet alone, according to G + C profiling of microbial populations in eight chicken farms in Southern Finland. This means that farmers may be able to eliminate the use of antibiotics for this purpose. The widespread use of antibiotics in food animals is linked to the development of antibiotic resistance in bacteria.
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