Article Abstract:
Fusel alcohols, formed by amino acid catabolism, induce the formation of hyphal-like extensions and pseudohyphae in yeasts indicating a close relationship between the formations of the two morphologies. Isoamyl alcohol induces the formation of hyphal-like structures in haploid and diploid strains of Saccharomyces cerevisiae at a concentration of 0.5% (v/v). A 0.25% (v/v) concentration of the alcohol produces pseudohyphae in S. cerevisiae. Isoamyl alcohol induces the formation of pseudohyphae in two Candida species and hyphal-like and pseudohyphal extensions in Brettanomyces anomalus.
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Article Abstract:
The process of ethanol-induced mycelial growth in Candida tropicalis was studied in conjunction with cytological characterization of actin localization. The use of ethanol resulted in biphasic growth curves. There was an accumulation of swollen spherical yeast cells during the first growth phase instead of the oblong ones found in control culture. Then, spherical daughter cells in chains appeared. Findings indicated that ethanol caused depolarization of cell growth during the first phase. Then the swollen cells grew as polar pseudohyphal cells.
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Article Abstract:
A study was conducted to analyze the antigenic features and cytolocalization of the Mycoplasma penetrans antigen, P35. Immunoelectron microscopy was carried out to show that P35 is exposed at the cell surface and distributed all over the membrane. Linear B-epitopes were then mapped using the ELISA approach based on a set of overlapping peptides. Results indicated a number of research implications concerning lipoprotein antigenicity during mycoplasma infections.
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