Article Abstract:
Research was performed to prove that the cell wall anchor of protein A from Staphylococcus aureus is functional in Lactococcus lactis. Evidence suggests that a heterologous anchor sequence can function in a highly-efficient manner to expose functional units at the surface of food-grade microorganism L. lactis. Further studies have to be undertaken as regards the biotechnological applications of this system in antigen presentation, immobilization of enzymatic groups at the surface and immobilization of production strains.
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Article Abstract:
Staphylococcus aureus Cowan I cells, expressing the Photinus pyralis luciferase (luc) gene, produce light which can be used for detecting antigen-antibody complexes by immobilizing the cells on a substrate containing immunoglobulin G. The cells produce a stable glow of light for almost 30 min in the presence of luciferase. The luc gene is present downstream of the S. aureus protein A promoter and fails to affect bacterial growth or plasmid stability.
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Article Abstract:
A comparative bioinformatic analysis of four publicly available Lactobacillus genomes and the genome of Lactobacillus salivarius subsp. salivarius strain UCC118 is performed to identify secreted proteins and those linked to the cell wall. The role in adhesion of the sortase protein and sortase-dependent proteins are examined by using transcriptional analysis and functional characterization of targeted gene knockout mutants.
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