Article Abstract:
The regions governing the replication, compatibility and maintenance of the Mycobacterium fortuitum plasmid pAL5000 were determined using a model organism, M. smegmatis strain mc(super 2)155. Two ORFs, repA and repB, were defined by deletion mutagenesis and subcloning. A 435 bp region which is needed in cis for replication was also defined. Cloning of this region (inc) into an unrelated mycobacterial replicon conferred incompatibility. Plasmids carrying this region had a reduced copy number due to changes in the plasmid structure. They were also less stable as compared to the wild-type species.
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Article Abstract:
Purification and inhibition by quinolones of DNA gyrases from Mycobacterium species with varying resistance to fluoroquinolones are discussed. Inhibition of the supercoiling activity of the purified enzymes was studied and compared to that for the DNA gyrase purified from E. coli. An extra mechanism, perhaps a low cell wall permeability or a drug efflux may contribute to the low antibacterial potency of quinolones in M. avium.
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Article Abstract:
A novel insertion sequence from Mycobacterium fortuitum has been isolated by using a trap vector developed out of inactivation of a lacZ reporter gene. The trap vector seems to be useful in trapping insertion sequences from different mycobacteria by screening for the disrupted LacZ phenotype. One of the predicted proteins had homology to what is said to be a transposase from Acetobacter pasteurianus.
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