RT-PCR as a tool for systematic transcriptional analysis of large regions of the Bacillus subtilis genome

Article Abstract:

Results demonstrate that reverse transcriptase-polymerase chain reaction technique can be used for simultaneous transcriptional analysis. Data also indicate that the technique does not involve hybridization and is sensitive.

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The Bacillus subtilis 168 csn gene encodes a chitosanase with similar properties to a Streptomyces enzyme

Article Abstract:

Research reveals that 168 csn gene encodes chitosanase as shown by the inability of a csn mutant to synthesize the enzyme in Bacillus subtilis. Enzyme kinetics and amino acid sequence data indicate that B. subtilis chitosanase bear close resemblance to that produced by Streptomyces species.

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Four genes encoding different type I signal peptidases are organized in a cluster in Streptomyces lividans TK21

Article Abstract:

Four clustered Streptomyces lividans TK21 genes which encode different type I signal peptidases (SPases) are discussed. Three of the genes make up an operon; the fourth is the first gene of another operon. Clustering of four different type I signal peptidase genes seems to be unique to Streptomyces among Gram-positive bacteria with multiple TypeI SPase genes.

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