Food-grade cloning and expression system for Lactococcus lactis

Article Abstract:

Stable and flexible vectors based on the high-copy-number endogenous Lactococcus lactis (L. lactis) pSH71 replicon, the lacF selection marker and the lactose-inducible lacA promoter have been developed. The nucleotide sequences of the vectors consist entirely of L. lactis DNA, suggesting that the vectors can be applied to lactococci improvement through self-cloning. This would improve lactococci's role as hosts for protein/peptide/metabolite production for the food industry.

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Use of green fluorescent protein to detect expression of an endopolygalacturonase gene of Colletotrichum lindemuthianum during bean infection

Article Abstract:

The Aequoria victoria green fluorescent protein was introduced to the 5' noncoding region of clpg2, an endopolygalacturonase gene of the bean pathogen Colletotrichum lindemuthianum. Results of the procedure as observed through fluorescence microscopy revealed that the expression of the gene clpg2 was expressed during the early stages of germination of the conidia. The same reaction was observed during in vitro and host plant's appressorium formation.

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Purification, characterization, gene cloning, sequencing, and overexpression of aminopeptidase N from Streptococcus thermophilus A

Article Abstract:

The characterization of the purified enzyme derived from aminopeptidase N is studied, since the enzyme is widely used in the manufacture of hard and semihard Swiss cheeses. Attempts were made to clone the pepN gene in a T7 promoter-based expression plasmid. The resulting 452-fold overproduced pepN enzyme was purified to homogeneity from the periplasmic extract of the culture host Escherichia coli strain.

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