Article Abstract:
The CBFB-MYH11 gene was introduced into mouse genome by inserting the 3' part of the human MYH11 cDNA into the end of exon 5 of the mouse Cbfb gene through homologous recombination. This led to the creation of a half genomic (Cbfb), half cDNA (MYH11) fusion gene, which, in turn, resulted in the generation of a Cbf(beta)-SMMHC fusion protein under the control of the Cbfb promoter. Cbf(beta)-SMMHC is shown to dominantly suppress the normal function of Cbf gene products and inhibits the differentiation of hematopoietic stem cells.
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Article Abstract:
The patterning of the ectoderm and mesoderm involves active BMP signaling on the ventral side and blocking BMP signaling on the dorsal side. This suggests that dorsal-ventral pattern formation may be a result of the presence or absence of BMP signaling. Further studies are recommended, especially on whether dorsal-ventral patterning of the endoderm also depends on BMP signaling as well as the nature of the molecule that initially induces mesoderm formation.
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Article Abstract:
Gene targeting was used to create mice completely deficient for Dv/1, a mouse homolog of the Drosphila segment polarity gene Dishevelled. The mice were viable, fertile and structurally normal, but showed reduced social interaction. Such mutants could therefore act as models for certain human psychiatric disorders.
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