Article Abstract:
Transcription of class II genes in yeast proceeds with the transcription factor (TF) IIIC (TFIIIC) binding to the promoter, then recruiting the TFIIIB to the TFIIIC-promoter complex. With the TFIIIB bound to the complex, RNA polymerase III (pol III) is then recruited for initiation to be activated. Recent researches showed that the RNA pol III directs multiple rounds of transcription. The stability of the TFIIIB-DNA complex that recruits the pol III into the initiation complex allows the pol III to act on the same template several times to complete several initiation cycles.
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Article Abstract:
The 5R mutant cell line was originally isolated as a cellular flat variant of Tax protein-transformed Rat-1 fibroblasts. The Tax protein is a transcription activator derived from the human T cell leukemia virus type 1 (HTLV-1). Characterization studies found 5R cells to be resistant to multiple NF-kappaB transcription factor activating stimuli, which made it ideal for use in the complementation cloning of the NF-kappaB Essential MOdulator (NEMO) gene. Results revealed that NF-kappaB activity is required for cell transformation.
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Article Abstract:
Single crystals of yeast RNA polymerase II without Tpb4 and Tpb7 have been grown for structural analysis and to test some ideas about the molecule. The result was structure determination to better than 6.0-angstrom resolution. Appropriate treatment os S-ray diffraction from an unoriented 18-heavy atom cluster derivative have significant phase information to 5-angstrom resolution. Some structure-function relationships which underlie the transcription mechanism have been suggested.
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