Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing

Article Abstract:

A kit method devised for purification of bacterial artificial chromosomes (BACs) could be adapted for effectively preparing individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria. A protocol is described for using solid-phase reversible immobilization (SPRI) for rapid, efficient and inexpensive isolation of sequencing-quality DNA of individual large, low-copy number plasmids from gram-negative and gram-positive bacterial strains.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Enantioselective synthesis of S-equol from dihydrodaidzein by a newly isolated anaerobic human intestinal bacterium

Article Abstract:

A chemically synthesized dihydrodaidzein was used as the substrate administered to a newly isolated reshaped, gram-negative, anaerobic bacterium from human feces, which can convert dihydrodaidzein to S-equol. Chiral stationary-phase high-performance liquid chromatography eluted only one metabolite, S-equol, which was produced form a bacterial culture containing a racemic mixture of dihydrodaidzein.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code:

Epoxide formation on the aromatic B ring flavanone by biphenyl dioxygenase of pseudomonas pseudoalcaligenes KF707

Article Abstract:

The first prokaryotic epoxide formation on the aromatic ring structures by biphenyl dioxygenase is presented. It suggests that different substrate configurations may react differently with high Fe oxo species of biphenyl dioxygenase.

User Contributions:

Comment about this article or add new information about this topic:

Comment:  (50-4000 characters)

Name:

E-mail:

Security Code: