Fab1p PtdIns(3)P 5-kinase function essential for protein sorting in the multivesicular body

Article Abstract:

A study was undertaken to examine the function of Fab1p gene in inhibiting vesicle formation and cargo selection within the endosome/multivesicular bodies (MVB) via the formation of the second messenger phosphoinositide, PtdIns(3,5)P2. Results revealed that PtdIns(3,5)P2 was produced by the conversion of the phosphatidylinositol 3-kinase by the PtdIns(3)P 5-kinase activity of the Fab1p gene. The synthesis of PtdIns(3,5)P2 was important in sorting membrane proteins into the lumen of the yeast vacuole observed in Saccharomyces cerevisiae.

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Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression

Article Abstract:

An essential gene from Saccharomyces cerevisiae, TOR2, whose product is proposed, on the basis of genetic evidence, to be the target for G1 arrest by FK506-binding protein12-rapamycin, was cloned and characterized. TOR2 exhibits significant homology with two previously identified phosphoinositide (PI) 3-kinases. The results suggest that 3-phosphorylated PIs may act as important signals for cell cycle activation.

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The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2

Article Abstract:

The mechanism by which TOR2 controls the actin cytoskeleton was examined using Saccharomyces cerevisiae phosphatidylinositol kinase homolog TOR2 as the model test system. The results confirmed that TOR2 sends signals to the actin cytoskeleton via a GTPase switch consisting of RHO1, RHO2, ROM2, and SAC7. The switch is activated by TOR2 through ROM2, presumably via the ROM2 PH domain.

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