Extensive in vitro hyphal growth of vesicular-arbuscular mycorrhizal fungi in the presence of carbon dioxide and flavonols

Article Abstract:

The effects of different flavonoids on the growth of Gigaspora margarita in pure culture were assessed. In the presence of two percent carbon dioxide, quercetin, myricetin and kaemferol stimulated growth. Hesperin was slightly inhibitory, while chrysin , biochanin A, flavone and apigenin were greatly inhibitory. Finally, chalcone stopped growth and naringenn and luteolin had no effect. The most extensive growth ever reported for a vesicular-arbuscular mycorrhizal fungus was achieved using quercetin with two percent carbon dioxide, which appeared toact synergistically.

author: Becard, G., Douds, D.D., Pfeffer, P.E.
Flavonoids, Bioflavonoids

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Laser microsurgery permits fungal plasma membrane single-ion-channel resolution at the hyphal tip

Article Abstract:

The application of laser microsurgery to Neurospora crassa hyphae for the formation of gigaohm resistance seals on the apical plasma membrane (PM) is described. The method allowed the resolution of single-ion-channel activity using patch clamp electrophysiology. Access to hyphal apex is permitted without the loss of morphological polarity and enzymatic cell wall digestion. The technique allows the full characterization of channels perceived to play an important role for tip growth.

author: Very, Anne-Alienor, Davies, Julia M.
Usage, Cell membranes, Plasma membranes, Lasers in surgery, Laser surgery, Neurospora

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Growth and mycorrhizal community structure of Pinus sylvestris seedlings following the addition of forest litter

Article Abstract:

Effects of addition of pine and oak litter on species composition and diversity of mycorrhizal fungi colonizing 2-year old Pinus sylvestris seedlings were investigated. Results provide evidence that the changes in supply of organic matter through litter manipulation has far reaching effects on chemistry of soil influencing growth and survival of Scots pine seedlings and their mycorrhizal community.

author: Aucina, Algis, Rudawska, Maria, Leski, Tomasz, Skridaila, Audrius, Riepsas, Edvardas, Iwanski, Michal
Physiological aspects, Company growth, Scots pine, Scotch pine

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subjects list: Research, Growth, Mycorrhizas, Mycorrhizae
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