Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A and B suggests a role in spermatogenesis and chromatin modification

Article Abstract:

The Saccharomyces cerevisiae RAD6 protein has been previously identified as an important component of the 'N-rule' protein breakdown pathway and in meiotic recombination. Cloning and expression of the human homologs of the RAD6 gene, designated HHR6A and HHR6B, revealed a highly conserved structure. However, UV induction in yeast was not preserved, indicating differences in the regulation of expression of these genes between yeast and mammals. Genetic analyses showed that HHR6 proteins are localized in euchromatin areas, suggesting their involvement in chromatin formation and spermatogenesis.

author: Hoeijmakers, Jan H.J., Willemsen, Rob, Hoogerbrugge, Jos W., Koken, Marcel H.M., Grootegoed, J. Anton, Jaspers-Dekker, Iris, Wit, Jan de, Roest, Henk P.
Chromatin, Saccharomyces

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Tissue-specific microtubule functions in Drosophila spermatogenesis require the beta2-tubulin isotype-specific carboxy terminus

Article Abstract:

Researchers have identified the degree to which a beta-tubulin isoform without its carboxy terminus can integrate into operational suprastructures by producing two early-stop-codon variants of the gene for the testis-specific beta-tubulin (beta 2) in Drosophila melanogaster. The carboxy terminus of beta 2-tubulin is essential for the alignment of microtubule suprastructures in spermatogenesis.

author: Fackenthal, James D., Turner, F. Rudolf, Raff, Elizabeth C.
Tubulins, Tubulin

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Molecular mechanism of nucleotide excision repair

Article Abstract:

The processes of nucleotide excision repair in DNA are set in motion through a specific molecular mechanism. This mechanism incorporates core factors which include damage sensors, catalyzers of open complex formation near lesions, damage binders, stabilizers and nuclease positioners, and incision catalyzers. Each component performs an important assembly-line function in DNA repair.

author: Hoeijmakers, Jan H.J., Jaspers, Nicolaas G.J., Laat, Wouter L. de
Netherlands, Models, Molecular biology, Nucleotides

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subjects list: Genetic aspects, DNA repair, Spermatogenesis, Research
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