Expression of the psl operon in pseudomonas aeruginosa PAO1 biofilms: PsIA performs an essential function in biofilm formation

Article Abstract:

The regulation of the psl gene cluster and the function of the first gene in the pslA gene cluster are investigated in biofilm formation and on the regulation of the entire psl operon in planktonic and biofilm cells. It is suggested that constitutive expression of the psl operon enables efficient attachment to surfaces and that regulated localized psl operon expression is required for biofilm differentiation.

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In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa

Article Abstract:

A study was conducted on the (14 super)C-labeled GDP-mannuronic acid, a substrate and subcellular fractions of alginate overproducing Pseudomonas aeruginosa FRD1 as a polymerase source, in the development of an enzymatic in vitro alginate polymerization assay. The results showed that a structural model of Alg8 was developed based on the structure of SpsA from Bacillus subtilis.

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Ps1D is a secreted protein required for biofilm formation by Pseudomonas aeruginosa

Article Abstract:

The function of ps1D, which is part of the psl operon from Pseudomonas aeruginosa, is investigated. An isogenic marker-free ps1D deletion mutant of Psuedomonas aeruginosa PA01 that is deficient in the formation of differentiated biofilms is generated.

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