Expression of rumen microbial fibrolytic enzyme genes in probiotic Lactobacillus reuteri

Article Abstract:

The cloning and expression of three rumen microbial fibrolytic enzyme genes in a strain of Lactobacillus reuteri is evaluated and the probiotic characteristics of these genetically modified lactobacilli is investigated. The L. reuteri transformed strains not only acquired the capacity to break down soluble carboxymethyl cellulose, bea-glucan, or xylan but also showed high adhesion efficiency to mucin and mucus and resistance to bile salt and acid.

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Colonization and immunomodulation by lactobacillus reuteri ATCC 55730 in the human gastrointestinal tract

Article Abstract:

Lactobacillus reuteri is a heterofermentive bacterium usually found in the gastrointestinal tract of humans as well as animals and it is also used as a dietary supplement. Studies were conducted to examine the local colonization of human gastrointestinal mucosa after dietary supplementation and it was found that dietary supplementation induced significant colonization of stomach and duodenum in humans as well as animals.

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The early response to acid shock in Lactobacillus reuteri involves the ClpL chaperone and a putative cell wall-altering esterase

Article Abstract:

The early response mechanism of Lactobacillus reuteri when it passes through the gastrointestinal tract was studied. The survival and gene expression of Lactobacillus reuteri after a sudden shift in environmental acidity and its early response involved the ClpL chaperone and a putative cell wall altering esterase.

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