Article Abstract:
A study was conducted to show that engineering the disulfide bond machinery of the cell through the expression of periplasmic cysteine oxidoreductases supported the production of active full-length tissue plasminogen activator (tPA). Results indicated several ways for increasing the expression of active, full-length tPA. Findings also correlated the oxidative folding of eukaryotic proteins in the periplasmic space with the isomerization of mismatched disulfides.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
A demonstration that low density lipoprotein receptor related protein 6 (LRP6), known to be a coreceptor for the Wnt signaling pathway, is required for anthrax toxin lethality in mammalian cells is presented. The results reported indicate that the LRP6 protein, which was known principally as a mediator of the Wnt signaling pathway, has a hitherto unsuspected role in the delivery of anthrax toxins into cells.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
Research demonstrates that the 17.4 kilodalton regulator of ribonuclease activity A, RraA, binds to ribonuclease E and inhibits the latter's endonucleolytic cleavages. Data indicate that RraA abundance circumvents an autoregulatory mechanism that maintains the cellular concentration of ribonuclease E leading to a genome-wide accumulation of ribonuclease E-targeted transcripts.
User Contributions:
Comment about this article or add new information about this topic: