Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae

Article Abstract:

The Trichoderma reesei beta-xylanase (XYN2) gene in Saccharomyces cerevisiae has been molecularly cloned. Multicopy plasmids using two Saccharomyces cerevisiae promoter-terminator expression cassettes aided in the expression of the XYN2 gene. Results supported the use of beta-cylanase for industrial enzyme production, even if its production by recombinant organisms m,ay not be as high as that in organisms such as the Trichoderma reesei strain.

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Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger beta-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes

Article Abstract:

Researchers successfully bioengineered Saccharomyces cerevisiae with the beta-xylosidase gene of Aspergillus niger 90196 and the beta-xylanase gene from Trichoderma reesei. The coproduction of these two fungal enzymes allowing the yeast to degrade xylan. Beta-xylanases have industrial applications in the food and biobleaching industries.

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Deletion of the GRE3 aldose reductase gene and its influence on xylose metabolism in recombinant strains of Saccharomyces cerevisiae expressing the xylA and XKS1 genes

Article Abstract:

Saccharomyces cerevisiae can create ethanol from xylose if it contains the xylA gene from Thermus thermophilus and the GRE3 yeast gene is removed. Xylose is a common sugar in lignocellulosic material, which is fermented to produce ethanol as an alternative to fossil fuels.

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