Expression of a Butyrivibrio fibrisolvens E14 gene (cinB) encoding an enzyme with cinnamoyl ester hydrolase activity is negatively regulated by the product of an adjacent gene (cinR)

Article Abstract:

The sequence of the second cinnamoyl ester hydrolase (CEH) encoding gene (cinB) isolated from ruminal bacterium Butyrivibrio fibrisolvens E14 was described. Sequence alignment allowed the identification of a possible helix-turn-helix DNA-binding domain. The lac promoter showed that cinR was present at high levels in Escherichia coli. Gel mobility-shift assays indicated that CinR bound specifically to cinR-cinB intergenic region. The role of Fara and FAXX suggested that CinB was likely to be involved in the degradation of plant materials by B. fibrisolvens.

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In situ detection of bacteria in continuous-flow cultures of seawater sediment suspension with fluorescently labelled rRNA-directed oligonucleotide probes

Article Abstract:

Natural bacterial populations in continuous-flow cultures of seawater sediment suspensions were studied using rRNA targeted and fluorescently labelled oligonucleotide probes. Around 40-80% of the DAPI-stained cells were detected with the EUB338 probe, and a shift in the composition of the natural bacterial population was also detected. The technique provides insights into the structure of populations growing in sediment suspensions.

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Bacillus subtilis ORF yybQ encodes a manganese-dependent inorganic pyrophosphatase with distinctive properties: the first of a new class of soluble pyrophosphatase?

Article Abstract:

Soluble inorganic pyrophosphatase is found in most living cells and it acts to remove pyrophosphate (PPi) generated by biosynthetic pathways. The identity of the Bacillus subtilis PPiase with the translation product of an ORF in the B. subtilis genome, has been identified. It appears unrelated to other PPiases but is similar to three other putative prokaryotic proteins. A new class of PPiase enzyme, type C, is proposed.

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