Article Abstract:
Two pseudomonad species designed to degrade substituted aromatic compounds were introduced into activated sludge microcosms to determine their survival, expression of engineered catabolic pathways and ability to transfer engineered plasmids. The results showed that the engineered bacteria could survive and grow under environmental conditions. They were also able to express their respective engineered catabolic pathways. In addition, in situ horizontal transfer of plasmid DNA was observed. The last observation has implications in the potential risk associated with the release of genetically-engineered microorganisms in the environment.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
A research was conducted to investigate the enzymatic reactions which contributed to the release of ammonia after ring cleavage where 2-aminomuconic semialdehyde was oxidized to 2-aminomuconate in the presence of NAD by enzymes in crude extracts. Pseudomonas pseudoalcaligenes was maintained using an agar-solidified, nitrogen-free medium while cells were suspended in a phosphate buffer. Results indicated that the reduction of nitrite to ammonia would require 3 mol of NAD(P)H if nitrobenzene serves as the nitrogen source for growth.
User Contributions:
Comment about this article or add new information about this topic:
Article Abstract:
A study of Pseudomonas pseudocaligenes and two other Pseudomonas strains shows that they can survive and grow in soil contaminated by 3-phenoxybenzoic acid and degrade the compound. The two strains completely degraded the compound when present in concentrations of only 30 CFU/g of soil.
User Contributions:
Comment about this article or add new information about this topic: