Article Abstract:
Results show that in wild type cells containing DnaA protein, the replication starts from orilambda and proceeds uni- and bi-directionally, whereas in DnaA deficient bacteria, replication of orilambda occurs only unidirectionally. Data further point out that this directionality is regulated by DnaA.
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Article Abstract:
A double mechanism of incompatibility between Escherichia coli dnaA(ts) host cells and lambda plasmids is discussed. A known plasmid host incompatibility seems to be the result of inefficient transcription stimulation from the p(sub.R) promoter by the mutant DnaA proteins, but a second is exemplified by the dnaA46 mutant. It is based on the competition between the lambda P protein and the host DnaA and DnaC proteins for DnaB helicase. Both mechanisms must be in effect for the incompatibility to exist.
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Article Abstract:
Experiments were performed to investigate the effects of Escherichia coli hosts harboring rpoA341 mutation on bacteriophage lambda lytic growth. Results showed that while there was no observed phenotypic differences between lambda lytic developments in rpoA+ and rpoA341 hosts growing in rich media, an effect was observed under slow growth conditons of the hosts. This finding can be explained by the ability of the phage lambda to produce an excess of certain proteins in rich media.
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