Examination of bacterial characteristics of anaerobic membrane bioreactors in three pilot-scale plants for treating low-strength wastewater by application of the colony-forming-curve analysis method

Article Abstract:

The characteristics of the bacterial populations of membrane bioreactors in three pilot-scale wastewater treatment plants were evaluated using the colony-forming-curve (CFC) method. The results showed that the viable populations of bacteria within the bioreactors included slow-colony-forming anaerobes, which could appear 10 days after incubation. In addition, most of the sludge anaerobes showed complex nutrient requirements. These results demonstrate the usefulness of the CFC method, although limitations such as the destruction of complex interactions among species and the difficulty of growing diverse sets of populations, should be recognized.

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Immunomagnetic detection of Bacillus stearothermophilus spores in food and environmental samples

Article Abstract:

A rapid assay for the detection of Bacillus stearothermophilus spores was developed based on immunomagnetic capture of the microbial spores from food. The rapid assay involved the utilization of a biotin-streptavidin complex and fluorescence detection. The immunomagnetically captured Bacillus stearothermophilus spores were also labeled with biotinylated-anti-BsS to minimize potential background created by nonspecifically bound organisms. Furthermore, the rapid assay was effectively utilized in the immunocapture of spores from a variety of complex backgrounds such as powdered milk, soil, sand and spices.

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Rates of inactivation of waterborne coliphages by monochloramine

Article Abstract:

The water quality from Denver's Direct Potable Reuse Demonstration Plant was evaluated by monitoring the presence of coliphages in the treated water. The results showed that some coliphages could escape the treatment process. Analysis of the contact time (CT) values of coliphage populations in the influent and treated water showed that escaping coliphages had higher CT values. This suggests that monochloramine treatment alone is not sufficient to remove coliphages with high CT values, specifically values exceeding 1,200.

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