Article Abstract:
Saccharomyces cerevisiae Spt4, Spt5 and Spt6 proteins seem to act as transcription factors that change chromatin form. Biochemical and genetic studies of these proteins show they have a role in transcription elongation. Conditional mutations in SPT5 that can be suppressed in an allele-specific way by mutations in the two largest subunits of RNA polymerase II (Pol II) have been isolated. One of the RNA Pol II mutants is defective for transcription elongation. The others cause phenotypes that would indicate an elongation defect.
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Article Abstract:
Mutation of the genes for SPT4, SPT5 and SPT6 of yeast alters transcription thereby suppressing mutations consisting of (delta) insertion at the LYS2 and HIS4 loci. Any two combination of mutations proved to be lethal for haploid yeasts and similar alteration in transcription was observed with mutations of all three. Coimmunoprecipitation studies revealed an interaction between at least SPT5 and SPT6. These findings imply that all the three have key roles in transcription and are necessary to retain viability in yeast.
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Article Abstract:
The SPT7 gene of Saccharomyces cerevisiae, a suppressor of delta and Ty insertion mutants in LYS2 and HIS4 genes, is necessary for proper transcription. The cloning and sequencing of SPT7 reveals that the gene is capable of encoding large acidic proteins localized to the nucleus. A bromodomain sequence which removes the bromodomain from the SPT7 protein constitutes the SPT7 protein in the SPT7 gene.
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