Evaluation of fluorescently labeled lectins for noninvasive localization of extracellular polymeric substances in Sphingomonas biofilms

Article Abstract:

Lectins may not be suitable for studying extracellular polymeric substances in biofilms. In a study of five fluorescently labeled lectins, only two bound to Spingomonas biofilms. This binding can not necessarily be used to identify specific target sugars in EPS.

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Use of fluorophore-conjugated lectins to study cell-cell interactions in model marine biofilms

Article Abstract:

The duatoms that synthesized several extracellular polymers, which can be differentiated after lectin staining and the significant interactions between diatoms and bacteria in mixed-species biofilms are reported. The active principle of the spent medium is probably a lectin or agglutinin that is able to blind to the extracellular plolymers of the diatoms involved in adhesion and motility.

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High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis

Article Abstract:

The conjugation rate among bacteria in a biofilm may be 1,000 times greater than among bacteria in a liquid culture. This was the conclusion of researchers who used automated confocal laser scanning microscopy to analyze conjugation in bacterial biofilms.

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