Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype Enteritidis in poultry
Article Abstract:
Research has been conducted on Salmonella enterica serotype Enteritidis. The authors describe the method for detecting this bacterium in poultry samples, and report that SYBR green I real-time PCR is an effective detection method.
author: De Medici, Dario, Croci, Luciana, Delibato, Elisabetta, Di Pasquale, Simona, Filetici, Emma, Toti, Laurs
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Italy, Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, Analysis, Physiological aspects, Environmental aspects, Genetic aspects, DNA, Microbiology, Salmonella, Methodology, Research methods, Microbial populations, Microbial ecology
Comparison of PCR, electrochemical enzyme-linked immunosorbent assays, and the standard culture method for detecting Salmonella in meat products
Article Abstract:
Two rapid techniques for detection of salmonella are evaluated. The standard culture method for detection of salmonella in food is rather sensitive and quite inexpensive, but it requires 4-5 days to generate results.
author: De Medici, Dario, Croci, Luciana, Delibato, Elisabetta, Volpe, Giulia, Palleschi, Giuseppe
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Salmonella typhimurium, Microbiological research
SYBR green real-time PCR method to detect Clostridium botulinum type A
Article Abstract:
SYBR green real-time PCR which was developed to detect Clostridium baratii and Clostridium butyricum type A revealing a very high specificity for the detection of C. botulinum types A and Ab is presented.
author: Aureli, Paolo, De Medici, Dario, Delibato, Elisabetta, Fenicia, Lucia, Anniballi, Fabrizio
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
Clostridium botulinum, Cyanides
subjects list: Research, Usage, Polymerase chain reaction
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