Article Abstract:
Two sets of independently regulated pectate lyase isozymes capable of macerating plant tissues were produced by Erwinia chrysanthemi EC16. Mutant CUCPB5012, which directs mutations in all the known enzymes in all clearing alpha-1,4 galacturonosyl linkages, is constructed to seek the unknown components of the pectolytic activity of E.chrysanthemi EC16. A novel set of pel isozymes in inoculated chrysanthemum cuttings and in minimal medium supplemented with crysanthemum cell walls is produced by the mutant which retains some ability to macerate chrysanthemum tissues.
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Article Abstract:
DNA sequencing of the Agrobacterium vitis pehA gene reveals a 1.6-kb open reading frame, which encodes a 549-amino-acid protein, which shows similarity to the polygalacturonases (PGs) of two other plant pathogens, Erwinia carotovora and Ralstonia solanacearum. The protein has a 34 amino acid N-terminal signal peptide and is an endo-PG. The A. vitis PG efficiently releases oligouronides from polygalacturonic acid, and is less damaging to potato tuber tissue than are the PGs from E. carotovora and R. solanacearum.
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Article Abstract:
Molecular analysis of 50 bacteriophage isolates of Erwinia amylovora from fire blight infected soil indicate that the isolates fall into six distinct groups. Electron microscopic analysis show distinct morphologies with Group 1 and 2 phages displaying tail and contractility and Groups 3 to 6 exhibiting short tails and thin appendages.
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