Epidemiological typing of Campylobacter isolates from meat processing plants by pulsed-field gel electrophoresis, fatty acid profile typing, serotyping, and biotyping

Article Abstract:

Four methods were used to conduct an epidemiological typing of Campylobacter spp. isolates that were identified as the leading causes of bacterial enteritis. Meat abattoir types were found to be related to different Campylobacter spp., where pork abattoir was linked to C. coli and C. jejuni with meat and poultry abattoirs. Pulsed-field gel electrophoresis was found to be the most efficient of the four methods. Serotyping is the second most effective followed by fatty acid profile typing and biotyping.

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Molecular analysis of the rfb O antigen gene cluster of Salmonella enterica serogroup O:6,14 and development of a serogroup-specific PCR assay

Article Abstract:

Research reveals that most of the O antigen biosynthetic enzymes are encoded by the rfb gene cluster in Salmonella. The gene cluster derived from a low G+C content organism possesses six open reading frames. Data indicate that O antigen factors O:24 and O:25 are encoded outside of the rfb gene cluster. The polymerase chain reaction (PCR) assay is developed based on the wzx gene.

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Use of pulsed-field gel electrophoresis and flagellin gene typing in identifying clonal groups of Campylobacter jejuni and Campylobacter coli in farm and clinical environments

Article Abstract:

Researchers used the flagellin gene of Campylobacter jejuni to show that many cases of human Campylobacter infection can be traced to farm animals. An estimated 2.4 million Americans develop diarrhea caused by Campylobacter jejuni every year.

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