Article Abstract:
Enzyme diffusion from Trichoderma atroviride (= T. harzianum P1) to Rhizoctonia solani is discussed. The diffusion has been found to be a prerequisite for triggering of Trichoderma ech42 gene expression before mycoparasitic contact. Results agree with those of enzyme diffusion from T. atroviride to R. solani generating the trigger of ech42 gene expression. It was found that cellophane membranes are permeable to proteins under 90kDa in size, at least, but dialysis membranes are not.
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Article Abstract:
A subgroup III G(alpha) subunit deletion mutant of Trichoderma atroviride is isolated and its role in the development and mycoparasitism is determined. The results show that the Trichoderma atroviride Tga3 has a general role in vegetative growth and can alter mycoparasitism-related characteristics, such as infection structure formation and chitinase gene expression.
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Article Abstract:
A reporter system based on the Aspergillus niger glucose oxidase was used to examine the regulation of expression of two major chitinase genes from the mycoparasitic biocontrol fungus Trichoderma atroviride. These are ech42, which encodes the CHIT42 endochitinase, and nag1, which encodes the CHIT73 N-acetyl-beta-D-glucosaminidase. Results demonstrate the difference in the mechanism of regulation of nag1 and ech42 expression. While nag1 is induced by low-molecular-weight chitooligosaccharides and its own catabolic products, ech42 seems not to be directly triggered by purified chitin or chitooligosaccharides, but by carbon starvation and some stress conditions.
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