Enzymatic activation of lysine 2,3-aminomutase from Porphyromonas gingivalis

Article Abstract:

An engineered lysine 2,3-aminomutase from Porphyromonas gingivalis, capable of acting on other L-amino acids, can function as a robust biocatalyst, depending on an activation system to trigger catalysis. It is demonstrated that in the absence of chemical reductants, a reducing protein such as flavodoxin [NADP.sup.+] reductase (FPR), flavodoxin (FLD) or ferrodoxin (FD) can activate lysine-2,3-aminomutase.

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Cloning and characterization of the xyl1 gene, encoding an NADH-preferring xylose reductase from Candida parapsilosis, and its functional expression in Candida tropicalis

Article Abstract:

Research describes cloning of Candida parapsilosis mutant strain KFCC-10875xyl1 gene encoding an NADH-preferring xylose reductase and its expression in the yeast Candida tropicalis, which is used in the industrial production of xylitol. The xylose reductase from C. parapsilosis exhibits a dual coenzyme specificity.

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Production of xylitol from D-xylose by a xylitol dehydrogenase gene-disrupted mutant of Candida tropicalis

Article Abstract:

Study is presented in which Ura-blasting method was used to sequentially disrupt two copies of the XYL2 gene encoding XDH in the diploid yeast Candida tropicalis. It is the first report on gene disruption of C. tropicalis for enhancing the efficiency of xylitol production.

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