Article Abstract:
The sulphur-oxidizing chemoautotrophic bacterial endosymbiont spreads to metamorphosed juveniles of the lucinid bivalve Codakia orbicularis through free-living symbiont forms probably present in the sand. Bacterium-specific and C. orbicularis-specific 16S rDNA primers amplify bacterial DNA in adult gills and metamorphosed juveniles cultivated in unsterilized sand, but not in adult ovaries, eggs and veligers or metamorphosed juveniles cultivated in sterilized sand. All adults host the bacteria in their gills, and juveniles cultivated in either sterile or unsterilized sand show similar growth. This indicates that the environmental transmission of the bacteria takes place after metamorphosis.
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Article Abstract:
Maintenance of pathogenicity of viable but nonculturable Salmonella typhimurium cells experimentally stressed with UV-C and seawater, was studied relative to the viability level of the cellular population. Pathogenicity, as tested in a mouse model, was lost concomitantly with culturability, while cell viability remained unaffected, as determined by respiratory activity and cytoplasmic membrane and genomic integrities.
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Article Abstract:
Single-cell approaches are used to investigate the characteristics of the sulfur-oxidizing symbiont of Codakia orbicularis. The results have helped in understanding the functioning of the symbionts within the host and also the host's role in controlling the growth of the symbionts.
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