Article Abstract:
The enumeration of phenanthrene-degrading bacteria is made easier by a new technique which involves adding the bacteria to agarose containing fine particles of phenanthrene and pouring this mixture onto a solidified agar underlayer. Counting the relevant colonies becomes easier due to the visible halo of phenanthrene disappearance around them. Applying this technique in the quantification of phenanthrene-degrading bacteria to various soils revealed that samples from soil contaminated with petroleum had more of these kinds of bacteria. Sites contaminated with phenanthrene-containing compounds had the most numerous colonies.
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Article Abstract:
A study was conducted to analyze the presence of anaerobic ferrous iron-oxidizing, nitrate-reducing bacteria in various European freshwater sediments. The number of prokaryotic cells were determined using the DNA-intercalating, blue fluorescent dye 4',6'-diamidino-2-phenylindole while ferrous iron concentrations were analyzed photometrically after chelation. Results indicated that enzymes involved in the anaerobic oxidation of ferrous iron can be utilized to effectively determine microbiological activities.
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Article Abstract:
The seasonal pattern of bacterial cotton fiber colonization was investigated. Dilution pour plating was used for the determination of the bacterial numbers of fiber samples while a modified dilution pour plating method was used for the characterization of gram-negative bacteria. Data were subjected to variance analysis and t tests. The growth of bacteria due to the rapid colonization of fiber was demonstrated.
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