Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry

Article Abstract:

Flow cytometry measures bacterial biomass by correlating the total fluorescence intensity with the dry weight of a sample of cells grown in a pure culture simulating an aquifer microcosm. Flow cytometry provides a superior method for enumerating bacteria as compared to conventional acridine orange direct counting. Flow cytometry can be used for a very large sample size, and needs only one-tenth of the time required by acridine orange direct counting. Both the methods give statistically similar results.

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The freeze-thaw stress response of the yeast Saccharomyces cerevisiae is growth phase specific and is controlled by the nutritional state via the RAS-cyclic AMP signal transduction pathway

Article Abstract:

A study was conducted on the conditions that promote the survival of yeast cells subjected to freeze-thaw stress. The factors examined involve signal transduction pathways, prior stress treatments, growth phase and mitochondrial functions. Results show that relationship between viability loss and the duration of freezing, suggesting that freeze-thaw damage is due mainly to freezing.

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A flow cytometry method for rapid detection and enumeration of total bacteria in milk

Article Abstract:

Researchers describe a flow cytometry method that clears milk enzymatically of milk proteins and lipids. This provides an accurate count of the bacteria in the milk and the procedure takes less than one hour.

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