Article Abstract:
Electrophoresis, Western blotting, Ent synthase assays, phenolate and protein assays and radioimmune precipitations were done to investigate the the localization and association of the enterobactin (Ent) synthase. Ent synthase, a product of the terminal reactions in the synthesis of the siderophore Ent by Escherichia coli which involve the EntD, E, F and B/G polypeptides, was found to interact with the cellular membrane in vivo. This indicate that the EntE, F and B/G are compartmentalized in the coliform bacteria and since other biosynthetic enzymes for siderophores and surfactants are associated with these Ent proteins, it appears that such class of proteins may be sequestered in vivo.
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Article Abstract:
Several studies were performed to determine the role of actin in the growth and hemolytic activity of Escherichia coli. Results revealed an increase in the culture supernatant hemolytic activity of E. coli grown in the presence of actin. Actin also elicited a rise in alkaline phosphatase activity in E. coli CC118. These findings confirmed the actin's influence in hemolysin production and pathogenesis in E. coli infections.
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Article Abstract:
A study was conducted to analyze the influence of hydrostatic pressure on ribosome conformation in Escherichia coli by differential scanning calorimetry. Experimental results indicated that the addition of MgCl2 before pressure treatment of cells hindered the reduction in stability of surviving ribosomes. However, the addition had no effect on the initial loss of enthalpy or on cell viability.
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