Article Abstract:
The ennoblement of stainless steel by the manganese-depositing bacterium Leptothrix discophora was studied to establish a chemical mechanism for ennoblement in which manganese dioxide acts as a galvanic cathode. The relationship among surface colonization, manganese deposition and open-circuit potential foe stainless steel coupons exposed to batch cultures of L. discophora were examined. Results reveal manganese-oxidizing bacteria are widely-distributed in nature and can cause several industrial problems.
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Article Abstract:
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis identifies an iron-oxidizing proteinwith an approximate molecular weight of 150,000. Different bacterial species tested for metal-oxidizing properties show that only Leptothrix discophora oxidizes both iron and manganese. A dot-blot assay developed to screen iron- oxidizing bacterial establishes different metal oxidizing properties for different bacterial species.
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Article Abstract:
The determination of procedures for isolating and maintaining sheath-forming strains of Leptothrix discophora in axenic culture is discussed. The manganese oxidizing activities of the strains were analyzed by microscopic localization, fractionation and electrophoresis. However, findings regarding the underlying genetic basis for the loss of the sheath-forming phenotype remains unclear.
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