Enhanced reactivity of Rhizopus oryzae lipase displayed on yeast cell surfaces in organic solvents: Potential as a whole-cell biocatalyst in organic solvents

Article Abstract:

Applications of genetically immobilized Rhizopus oryzae lipase displayed on the cell surface of Saccharomyces cerevisiae in organic solvents and measured the catalytic activity of the displayed enzyme as a fusion protein with alpha-agglutinin is evaluated. The results show that the yeast cells displaying the lipase can be used in organic solvents and the lipase activity may be increased by a combination of protein engineering and display techniques.

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In vivo synthesis of mammalian-like, hybrid-type N-glycans in pichia pastoris

Article Abstract:

An engineering of the P. pastoris N-glycosylation pathway to produce nonhyperglycosylated hybrid glycans are described. This is done by inactivation of OCH1 and over expression of an alpha-1,2-mannosidase retained in the endoplasmic reticulum and N-acetyglucosaminyltransferase I and beta-1,4-hybrid-type N-linked oligosaccharide structure on its glycoproteins.

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Enhanced production of alpha-galactosyl epitopes by metabolically engineered Pichia pastoris

Article Abstract:

Research has been conducted on Pichia pastoris. The authors describe the construction of metabolically engineered P. pastoris system for the large-scale production of alpha-galactosyl epitopes with in situ UDP-galactose regeneration.

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